Background: Running has become a very popular mode for exercise in the United States. Unfortunately, many runners become injured as they train for long distance races. Developing a pre-training screen that predicts running injury would be beneficial. Purpose: The purpose of this study was to determine if a select pre-training screen consisting of great toe extension, lower extremity reaches and thoracic spine mobility is associated with development of injury in runners training for a long distance race. Subjects: Twenty-two runners were recruited and qualified for the study. Seven runners were lost to attrition- resulting in 15 runners (11 females, 4 males) between the ages of 21-45 who were training for either a half or full marathon that participated in the study. Methods: Demographic data including age, gender, height, weight, and body mass index (BMI) were collected from all participants. In addition, great toe extension, combined thoracic rotation and sidebending, and multiplane lower extremity reaches were measured on runners prior to beginning their 12-week training program for a half or full marathon race. Each runner was asked to log their weekly training mileage and record any injury that may have occurred during the 12 weeks. Mean and standard deviation were calculated for each outcome measure. Independent t-tests were calculated for the three outcome measures between injured and non-injured groups. Alpha level was set at p ≤ 0.05. Results: Eleven of the fifteen runners did not incur injury during their training. Four of the fifteen experienced some type of lower extremity injury during their training. There was no statistically significant difference in the injured versus non-injured runners with regards to the outcome measures. However of interest, the injured runners were all females, slightly older (35.23 years), and had higher body mass (28.44).